Device
Part:BBa_K3264024:Design
Designed by: Diol Wang Group: iGEM19_GreatBay_SZ (2019-10-16)
TALEsp-vioA-vioB-vioC-vioE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1513
Illegal NheI site found at 1921
Illegal NheI site found at 4184 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 113
Illegal XhoI site found at 2152
Illegal XhoI site found at 2692 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6966
Illegal AgeI site found at 747
Illegal AgeI site found at 849
Illegal AgeI site found at 1767
Illegal AgeI site found at 2277
Illegal AgeI site found at 7162 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
A TALE stabilized "constitutive" promoter generate enzyme vioA, vioB, vioC, and vioE. This part is working on a low copy number pSC101 plasmid.
Source
Composite part, sources are come from iGEM part collection.